CHLOROQUIN INACTIVATES LYSOSOMAL FUNCTION(S) IN NORMAL CULTURED HUMAN FIBROBLASTS
نویسندگان
چکیده
منابع مشابه
Lysosomal cystine storage augments apoptosis in cultured human fibroblasts and renal tubular epithelial cells.
Nephropathic cystinosis is a lethal disorder of lysosomal cystine storage due to defective lysosomal cystine transport. How lysosomal cystine causes this multisystemic disorder culminating in end-stage renal disease is not known, because the cystine is isolated from cellular metabolism by the lysosomal membrane. It is here reported that in both normal and nephropathic cystinotic fibroblasts and...
متن کاملRecognition and receptor-mediated endocytosis of the lysosomal acid lipase secreted by cultured human fibroblasts.
We have studied the recognition and uptake of acid lipase by human fibroblasts in order to determine requirements for localization and function of the enzyme in lysosomes. Our approach was based on evidence that a number of acid hydrolases involved in mucopolysaccharide metabolism are secreted from cultured fibroblasts and endocytosed by a phosphomannosyl-dependent, receptor-mediated process. A...
متن کاملDynamics of lysosomal cholesterol in Niemann-Pick type C and normal human fibroblasts.
The dynamics of endolysosomal cholesterol were investigated in Niemann-Pick type C (NPC) cells and in human fibroblasts treated with class 2 amphiphiles to mimic NPC cells. We showed through new approaches that the massive pools of endolysosomal cholesterol in these cells are not trapped but, rather, circulate to the cell surface at about the normal rate. This flux spared NPC and amphiphile-tre...
متن کاملSecretion of lysosomal enzymes by normal and cystic-fibrosis fibroblasts
The secretion of cellular components by mononuclear phagocytes, lymphocytes and neutrophils seems to play an important role in the inflammatory process (Davies & Allison 1976). It is likely that the secretory activity of one cell will be modified by the activities of the others. Since the macrophage is the predominant cell in chronic inflammation, we studied its lysosomal enzyme secretion and i...
متن کاملDihydrotestosterone Formation in Cultured Human Fibroblasts
The conversion of [1,2-3H]testosterone to [‘Hldihydrotestosterone has been assessed in fibroblast monolayers grown from skin biopsies of foreskin, scrotum, and various nongenital skins from 31 control men who varied in age from newborn to 25 years and three 46,XY subjects with hereditary male pseudohermaphroditism. Under the standardized conditions utilized in this study, the rate of dihydrotes...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Pediatric Research
سال: 1974
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-197402000-00037